About
On this website you will find different surface protein expression datasets exploring CD8+ T cell heterogeneity following infection with various pathogens. These datasets have been generated in the laboratory of Laura Mackay using the InfinityFlow analysis pipeline (Becht et al. 2021 https://dx.doi.org/10.1126/sciadv.abg0505). Here, you would be able to interrogate the expression of hundreds of surface proteins on antigen-specific CD8+ T cell across different tissues.
Datasets
P14 memory CD8+ T cells across 6 tissues (LCMV Armstrong 60 days post-infection)
We examined the surface proteome of antigen-specific CD8+ T cells (P14) across six different tissues (spleen, bone marrow, liver, kidney, salivary glands, small intestine IEL) following an acute viral infection (LCMV Armstrong, 60 days post- infection). For this, we used the InfinityFlow analysis pipeline and the LEGENDScreen antibody panel (Biolegend), which allows for the measurement of hundreds of surface proteins at the single-cell level. CD8+ T cells were segregated into distinct populations based on InfinityFlow predicted protein expression. We present these analyses for interactive browsing. Further information can be found in our publication: insert DOI when available.
P14 circulating memory CD8+ T cells (LCMV Armstrong 60 days post-infection)
We examined the surface proteome of antigen-specific CD8+ T cells (P14) in the spleen following an acute viral infection (LCMV Armstrong, 60 days post- infection). For this, we used the InfinityFlow analysis pipeline and the LEGENDScreen antibody panel (Biolegend), which allows for the measurement of hundreds of surface proteins at the single-cell level. Circulating memory CD8+ T cells were segregated into distinct populations (TCM, TEM1, TEM2 and t-TEM) based on InfinityFlow predicted protein expression. We present these analyses for interactive browsing. Further information can be found in our publication: insert DOI when available.
OT-I circulating memory CD8+ T cells (LCMV-OVA and Influenza-OVA 20 days and 100 days post-infection)
We examined the surface proteome of antigen-specific CD8+ T cells (OT-I) in the spleen following an acute viral infection (LCMV-OVA or Influenza-OVA (X31), 20 days and 100 days post-infection). For this, we used the InfinityFlow analysis pipeline and the LEGENDScreen antibody panel (Biolegend), which allows for the measurement of hundreds of surface proteins at the single-cell level. Circulating memory CD8+ T cells were segregated into distinct populations (TCM, TEM1, TEM2 and t-TEM) based on InfinityFlow predicted protein expression. We present these analyses for interactive browsing. Further information can be found in our publication: insert DOI when available.
OT-I memory CD8+ T cells across 7 tissues (LCMV-OVA, Listeria-OVA, HSV-OVA and Influenza-OVA 50 days post-infection)
We examined the surface proteome of antigen-specific CD8+ T cells (OT-I) in the spleen following an acute infection (LCMV-OVA, Listeria-OVA, HSV-OVA or Influenza-OVA (X31), 50 days post-infection). For this, we used the InfinityFlow analysis pipeline and which allows for the measurement of surface proteins at the single-cell level. Memory CD8+ T cells were segregated into distinct populations (TCMEM, TCEFF, and TRM) based on InfinityFlow predicted protein expression. We present these analyses for interactive browsing. Further information can be found in our publication: insert DOI when available.